DNA damage, homology-directed repair and DNA methylation
نویسندگان
چکیده
منابع مشابه
DNA Damage, Homology-Directed Repair, and DNA Methylation
To explore the link between DNA damage and gene silencing, we induced a DNA double-strand break in the genome of Hela or mouse embryonic stem (ES) cells using I-SceI restriction endonuclease. The I-SceI site lies within one copy of two inactivated tandem repeated green fluorescent protein (GFP) genes (DR-GFP). A total of 2%-4% of the cells generated a functional GFP by homology-directed repair ...
متن کاملCorrection: DNA Damage, Homology-Directed Repair, and DNA Methylation
[This corrects the article DOI: 10.1371/journal.pgen.0030110.].
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In this work, we examine regulation of DNA methyltransferase 1 (DNMT1) by the DNA damage inducible protein, GADD45α. We used a system to induce homologous recombination (HR) at a unique double-strand DNA break in a GFP reporter in mammalian cells. After HR, the repaired DNA is hypermethylated in recombinant clones showing low GFP expression (HR-L expressor class), while in high expressor recomb...
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DNA repair is essential to maintain genomic integrity and initiate genetic diversity. While gene conversion and classical nonhomologous end-joining are the most physiologically predominant forms of DNA repair mechanisms, emerging lines of evidence suggest the usage of several noncanonical homology-directed repair (HDR) pathways in both prokaryotes and eukaryotes in different contexts. Here we r...
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ژورنال
عنوان ژورنال: PLoS Genetics
سال: 2005
ISSN: 1553-7390,1553-7404
DOI: 10.1371/journal.pgen.0030110.eor